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phosphonf kb  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology phosphonf kb
    Phosphonf Kb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fig. 5 Expression of phosphorylated NFkB p65 shows an increased trend in macrophages after stimulation with glycated soy. Expression of phosphorylated NFkB P65, analyzed by western blot (A) THP-1 differen- tiated into macrophages, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy for 10 minutes, data is representative of 3 replicates. (B) Primary derived adherent monocytes from one donor, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy, data is representative of two replicates. Quantified data based on the adjusted volume normalized to beta actin. Data was processed and quantified using the ImageLab software from BioRad.

    Journal: Food & function

    Article Title: Characterization of different stages of Maillard reaction in soy: impact on physicochemical properties and immunogenicity of soy proteins.

    doi: 10.1039/d4fo04400b

    Figure Lengend Snippet: Fig. 5 Expression of phosphorylated NFkB p65 shows an increased trend in macrophages after stimulation with glycated soy. Expression of phosphorylated NFkB P65, analyzed by western blot (A) THP-1 differen- tiated into macrophages, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy for 10 minutes, data is representative of 3 replicates. (B) Primary derived adherent monocytes from one donor, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy, data is representative of two replicates. Quantified data based on the adjusted volume normalized to beta actin. Data was processed and quantified using the ImageLab software from BioRad.

    Article Snippet: The membrane was then washed 3×, followed by incubation with primary antibody (PhosphoNF-κB p65 (Ser536) (93H1) Rabbit mAb, Cell Signaling) overnight at 4 °C.

    Techniques: Expressing, Western Blot, Derivative Assay, Software